Nanopore sensors read individual molecules, one at a time, using electrical sensing. No optics. No chemistry.
Single molecule “event” has depth and duration, proportional to its size.
Events are plotted based on impedance (depth) and duration.
Detecting Target Analytes
2PG's reagent compound
- Bulk-phase association kinetics in 3D volume is faster and more efficient than 2D (surface) binding in ELISA tests.
- 2PG’s reagent is a synthetic DNA scaffold tethered to a target analyte binding domain.
- When scaffolds pass through the nanopore, they create discrete impedance signatures.
Types of Binding Molecules
- Fab fragments
- Full length antibodies
Test: Target analyte (HIV antibody) spiked into saliva at clinical concentrations (nM)
Target is spiked into saliva and incubated with detection reagent, and read directly by nanopore sensor. No prep necessary.
Events are plotted based on (impedance) depth and duration